Flow Cytometry: Methods And Protocols | Imaging

: Beyond fluorescence, IFC provides data on cell size, shape, circularity, and internal complexity.

: Non-specific binding is prevented using anti-Fc antibodies. Cells are then stained with fluorophore-conjugated antibodies, viability dyes, or nuclear dyes like DAPI or Hoechst to provide spatial landmarks. Imaging Flow Cytometry: Methods and Protocols

While IFC shares many preparatory steps with conventional flow cytometry, it requires specific optimizations to ensure high-quality imagery. : Beyond fluorescence, IFC provides data on cell

: Cells must be in a single-cell suspension to avoid clogging the thin fluidic channels used for imaging. While IFC shares many preparatory steps with conventional

: Researchers must set specific software parameters, such as magnification and laser power, to maximize the resolution of the captured images. Common Applications

IFC provides a "visual verification" for every data point, allowing researchers to see exactly what their gated populations look like.