This technology has been instrumental in recent studies, including identifying SARS-CoV-2 protease cleavage sites and characterizing human cannabinoid receptors.
A C4 ZipTip is a standard pipette tip containing a microscopic bed of resin fixed at its distal end. Unlike C18 resins, which are used for smaller peptides, the C4 resin is optimized for larger, more hydrophobic molecules like proteins and large polypeptides. Primary Uses in Research Researchers use these tools for two main reasons: c4.zip
: Contaminants are washed away with a dilute acid, such as trifluoroacetic acid (TFA) . This technology has been instrumental in recent studies,
: The purified protein is eluted directly onto a target plate using an organic solvent like acetonitrile , where it is mixed with a matrix for analysis. Key Specifications Resin Type : Butyl (C4) silica. Primary Uses in Research Researchers use these tools
The C4 ZipTip is most commonly used in the workflow for (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight).
Expression, Purification and Characterization of the Human ... - Nature